Gil Yosipovitch
The purpose of this research is to understand the biological regulation mechanism of 5-hydroxymethylcytosine (5hmC) formation in distinct murine brain regions during aging. Numerous studies demonstrate the enrichment of 5hmC in brain. Being the intermediate in Deoxyribonucleic Acid (DNA), demethylation 5hmC it is also regulated by certain chromatin structures. As there is an unknown biological mechanism in brain we investigated the dynamics of H3K4me3 and H3K27me3 modifications for Ten-eleven translocation (Tet) gene expressions and for the known quantitative 5hmC levels by focusing distinct developmental status (embryonic day 16, day of birth, day 7, 15, 30 and 120 after birth) of frontal and cerebellar cortex in murine brain. In this research, we summarize the findings in availability of Tet gene and of 5hmC, and their dependence on transcriptional regulation at H3K4 and H3K27.Furthermore, we discuss future challenges and directions.