医薬品分析化学: オープンアクセス
オープンアクセス

概要

On-line SPE of Three Steroids with a Novel Polymeric Monolith

Haiyan Liu, Hang Zhang, Shuai He, Haonan Li and Ligai Bai

In this report, a novel poly (N-isopropyl acrylamide-glycidyl methacrylate-ethylene glycol dimethacrylate) monolithic column was introduced for solid phase extraction. The monolith was prepared using N-isopropyl acrylamide and glycidyl methacrylate as the functional monomers, ethylene glycol dimethacrylate as the cross linker. In order to evaluate the mechanical stability and permeability of the material, the pressure drop across the column was measured in different flow-rates. Moreover, the monolith was used as on-line solid phase extraction material and coupled to high performance liquid chromatography to determine dexamethasone acetate, norgestrel and halcinonide in urine samples.