プロテオミクスとバイオインフォマティクスのジャーナル
オープンアクセス

概要

Quantitative Analysis of Cytokines in Diabetic Nephropathy

Srinubabu Gedela, Venkata Phani Bhushan Guruju, Mary Sulakshana, Inavolu Lakshmi Maheswari, T. Prabhakar, GirijaSankar, Allam Appa Rao and GR Sridhar

Diabetes mellitus is the most important public health problems due to its high prevalence and enormous social and economic consequences. Diabetic nephropathy is one of the chronic complications of diabetes mellitus. Inflammatory mediators are believed to play a vital role as predictors of low-grade systemic inflammation in diabetic nephropathy. Pro- and anti-inflammatory mediators relationship between ageing and low-grade systemic inflammation could be determined by measuring pro- and anti-inflammatory mediator’s presence in the circulation in subjects of different age groups.

To examine the impact of inflammatory mediators on diabetic nephropathy, we quantified important inflamma- tory mediators such as CRP, IL-10, IL-6, MPO and TNF-α in diseased and controls using standard ELISA assay. Correlation between the multiplexed assays of ELISA was good for CRP, IL-6, IL-10, TNF-α and myeloperoxidase. Within and between run impression values for the multiplex method was < 15%. As an easier and cheaper test for assessment of diabetic nephropathy, we here with recommend further studies of CRP in diabetic patients.