応用微生物学: オープンアクセス
オープンアクセス

概要

Simultaneous Determination of Omeprazole, Tinidazole and Clarithromycin in Bulk Powder and Helicure Tablets by HPLC

Hesham Salem, Safa M Riad, Mamdouh R Rezk and Kholoud Ahmed

Sensitive and precise chromatographic method was developed and validated for simultaneous determination of omeprazole (OMP), tinidazole (TND) and clarithromycin (CLR) in bulk powder, laboratory prepared mixture and pharmaceutical preparation. The technique adopted for quantification is HPLC. A mixture of acetonitrile, methanol, phosphate buffer at pH 3.5 (33: 17: 50, v/v/v) was used as a mobile phase. The stationary phase used was (150 mm×4.6 mm, 10μm) C8 LichrosorbTM analytical column. The method was linear in the range of 0.2-250 μg mL-1, 0.5-250 μg mL-1 and 75-2000 μg mL-1 for OMP, TND and CLR respectively. The selectivity of the proposed method was checked using laboratory prepared mixtures. The proposed method was successfully applied to the analysis of OMP, TND and CLR in their mixture and in pharmaceutical dosage form without interference from other additives