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概要

Genomics 2019: Transgenic Mice Brain Imaging Studies of Alzheimer

Chen Jenn-Tzong

Lipophilicity is one of the significant mind radiopharmaceutical plan rules. Alzheimer sickness PET imaging specialists dependent on lipophilicity change are [18F]RO6958948 [1] and[18F]Florbetapir, plan by supplanting with a Nitrogen component either in the fragrant ring of [18F]Flortaucipir or [18F]Florbetaben. The structure of [18F]FEONM (Figure 1) is intended to give higher lipophilicity than [18F]FDDNP. Structure alteration on a specific bioactive atom to expand its lipophilicity will be likewise potentially expanding the level of infiltrating blood cerebrum obstruction. Expanding the blood cerebrum obstruction crossing proportion, the particularity of this dynamic biomolecule focusing on impact may be diminished. Along these lines, we plan an ethyl oxide changed naphthol based Alzheimer infection positron outflow tomography imaging specialist [18F]FEONM, to think about the take-up impact of Tau tangle and Beta amyloid.

 

PET radiopharmaceiticals for mind imaging depend on extremely short half-life radionuclides, the vast majority of them will be rotted in one day. One of the longest half-life natural radionuclides is fluorine-18, in this way basic advance to creating PET radiopharmaceuticals online is radiofluorination response. The most elevated radiofluorination response yield can be produced using carboxy glass reactor. In carboxy glass reactor, the capacity of hole territory (FG) bend of radiofluorination yield can be drawn nearer with Gauss dispersion, Gauss or Welch apodization work. After decide the radiofluorination rate consistent, the length of microfluidic plug stream reactor can be planned with an expository structure dependent on Welch apodization work.

 

Mind hippocampus imaging relative explicit restricting proportion of [18F]FEONM on a Tau tangle P301S/PS19 transgenic mouse model is double cross higher than cerebellum, Beta amyloid Tg2576 transgenic mouse model is under two. On a triple transgenic 3xTg mouse model with both Tau tangle and Beta amyloid framed, the take-up proportion of hippocampus is 50% higher than cerebellum. Consequently, [18F]FEONM is another Alzheimer PET imaging specialist. In addition, other than transgenic mouse model, streptozotocin actuated Tau tangle mouse model likewise shows higher cerebrum hippocampus [18F]FEONM take-up than control mouse.

 

From the transgenic mouse model imaging study, we discovered [18F]FEONM will take-up on both Tau tangle and Beta amyloid transgenic mouse. In contrast with [18F]FDDNP, it shows no Beta amyloid transgenic mice take-up in mind hippocampus. This outcome speaks to part of the particular authoritative of Tau tangle transgenic mouse of [18F]FDDNP has move to Beta amyloid. In this manner, Tau tangle and Beta amyloid take-up status should be possible by [18F]FEONM in a similar time for conclusion Alzheimer illness. Radiation presentation will be half measurement contrasted with taking both imaging. These discoveries dependent on another plan presume that another PET radiopharmaceutical configuration has a similar idea like another radiofluorination microfluidic reactor plan. Either another synthetic structure or another numerical model contributes an accomplishment. For instance, an undeniable application is assessing helpful adjustment of sickness movement. At the finish of this survey, the creators incorporate outcomes from a pilot study exhibiting achievability of utilizing MRMI to identify restorative change of plaque movement in AD transgenic mice. Despite the species picked, transgenic advancements present hereditary changes. In this way, fruitful demonstrating requires the sickness to be related with a hereditary change or if nothing else for a theory to exist in regards to the presumable pathophysiology of the problem that can be displayed by a hereditary adjustment. To be helpful as a creature model, the transgenic living being must likewise have the option to show the fundamental obsessive, physiological, or conduct highlights of the human sickness.

With quality focusing, rather than an unfamiliar transgene being presented, an endogenous quality in the mouse is adjusted. At first, the adjustment is made in particular cells named undeveloped stem (ES) cells. ES cell lines are gotten from beginning phase mouse undeveloped organisms and can be kept up uncertainly in an undifferentiated state in vitro yet hold the limit, when infused once more into a beginning phase mouse incipient organism, to blend in with the endogenous cells of the incipient organism and add to all tissues of the creating mouse, including the germ line. The quality of interest is altered in ES cells by the presentation of a focusing on vector that comprises of an adjusted form of the endogenous quality. In ES cells, the focusing on vector recombines with the homologous endogenous quality and accordingly presents the hereditary adjustment. Quality focused on ES cells are then infused into wild sort blastocyst-stage mouse undeveloped organisms with the fanciful mice that outcome being combinations of the changed ES cells and wild sort blastocyst cells. The effective joining of the ES cells into the germ line allows the hereditary alteration to be proliferated as a feature of the mouse genome, and this makes stable transgenic lines.